This dissertation thesis was designed to contribute to a better understanding of isolation, proliferation and differentiation of dental stem cells (DSCs) derived from human teeth under specific treatment conditions for bone regeneration. The extracted impacted third molar teeth are discarded source of human waste, which harbor tissues that have potential stem cells. The dental tissues that can be obtained from the teeth are dental pulp tissue, dental follicle tissue and periodontal tissue. These tissues can be enzymatically digested to isolate cells; DSCs obtained from all three tissues proliferated and could be differentiated osteogenically in our studies. The advantage of these cells over the more standard, more clinically developed mesenchymal stem cell (MSC) source derived from bone marrow tissues (BM-MSCs) is that DSCs can be obtained from discarded teeth from routine dental procedure, with low tissue site morbidity and the cell numbers obtained are higher.